Coding

Part:BBa_K4244008:Design

Designed by: Arthur Vancolen   Group: iGEM22_Wageningen_UR   (2022-10-08)


Dismed2EnvZ chimeric sensor of two component system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 271
    Illegal PstI site found at 804
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 271
    Illegal PstI site found at 804
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 271
    Illegal PstI site found at 804
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 271
    Illegal PstI site found at 804
    Illegal NgoMIV site found at 69
    Illegal NgoMIV site found at 88
    Illegal NgoMIV site found at 535
    Illegal NgoMIV site found at 1002
    Illegal NgoMIV site found at 1042
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

Amplify from E.coli Nissle 1917 and Pseudomona Areuginosa

References